You got your order

Water line

Water delivery — how much apple cider vinegar for chickens: • General use: 1 oz per gallon of drinking water • Stress events (molt, heat, transport): 2 oz per gallon for 3-5 days • Maintenance: 1 oz per gallon, 3-4 days per week Tips: • Use a plastic waterer — ACV corrodes galvanized metal • Best results when introduced before stress events • Shake well before use — settling is normal for raw ACV

Water — keep Mother Load ACV 3 days apart from Hen Helper

Hen Helper: • 1 tablespoon per gallon of drinking water • Use daily for the first 2 weeks, then 3-4 times per week Mother Load ACV: • 1 tablespoon per gallon of drinking water • Use 3-4 times per week as ongoing maintenance • Increase to daily during stress (molting, extreme weather) • Space it at least 3 days apart from Hen Helper (see tip below) Catalyst Vitamins: • Follow label directions for flock size • Use 2-3 times per week for maintenance • Use daily during molting, illness recovery, or extreme weather Tip: Hen Helper and Catalyst can go in the same waterer together. Mother Load ACV is a different type of probiotic than Hen Helper, so keep those two at least 3 days apart — running them together cancels out the beneficial microbes. Catalyst is a vitamin and mixes with anything, anytime.

Water line (medicator or proportioner)

Standard protocol (broilers): • Days 1-3: 1 oz per 100 gallons (heavy dose for gut colonization) • Days 4-14: 0.5 oz per 100 gallons (maintenance) • Day 15+: 0.5 oz per 100 gallons every 3rd day Layers / Turkeys: • First 14 days: 1 oz per 100 gallons daily • Maintenance: 0.5 oz per 100 gallons 2x/week Stress events (heat, transport, vaccination): • Return to heavy dose for 3 days

Water line (medicator or proportioner), or mixed directly into drinking water

Commercial poultry (medicator): • 100 CC (2 scoops) per 5 gallons of stock solution • Medicator pump set at 1:128 • Start chicks on Catalyst day 1, running for 1–2 days • Then run once per week for 24 hours Backyard poultry: • ¼ teaspoon per gallon of fresh drinking water • For greater small-scale efficiency, use the Catalyst Liquid Formula For best results, run Catalyst alongside Big Ole Bird Poultry Probiotics.

Drinking water (waterer or nipple system)

Standard dosing: • Add 1 tablespoon per gallon of drinking water • Replace treated water every 2-3 days Stress events (heat, cold, molting, new birds): • Double dose for 5-7 days, then return to standard Chicks: • Start from day one at standard dose • Supports strong early development

Pump sprayer or boom sprayer

Commercial broiler house — cleanout (between flocks): • Mix 1 gallon concentrate per 20 gallons of water • Spray litter, walls, posts, and under equipment • A standard 40×500 house requires 2-3 gallons concentrate per cleanout • Allow to dry before placing new bedding Commercial house — during production: • Mix 1 gallon concentrate per 30 gallons of water • Spray perimeter walls, posts, and beetle congregation areas • A standard 40×500 house requires 1-2 gallons concentrate for perimeter treatment • Avoid direct contact with feed and water lines Layer and breeder houses: • Same dilution rates as broiler houses • Focus on pit areas, under slats, and manure belt areas • Treat monthly during peak beetle season Maintenance: • Reapply every 2-3 weeks during heavy infestations • Monthly maintenance spray during lower pressure periods

Drinking water (waterer or nipple system)

Standard dosing: • Add 1 tablespoon per gallon of drinking water • Replace treated water every 2-3 days (or as waterer is refilled) New chicks: • Start on day one for best results • Same dose — 1 tablespoon per gallon Stress events (extreme heat, new birds, predator scares): • Double dose for 3-5 days, then return to standard

Pump sprayer, boom sprayer, house washer, or ATV tank — any standard farm sprayer

Mix ratio: • 1 gallon Litter Life + 9 gallons water per 2,000 sq ft • 20,000 sq ft house = 10 gallons concentrate (mixed with 90 gallons water) • Optional: let the mix sit 1–24 hours before spraying Timing (before placement): • Apply 7–10 days before placement (best: 12–14 days) • Best on a bare pad; if not possible, apply on built-up litter before new bedding goes down • Close the house for 1–2 days after spraying to hold warmth, then ventilate Heavy ammonia: double the application rate.

Direct spray application

Surface sanitation: - Spray surface until thoroughly wet - Allow to air dry (no wiping or rinsing needed) - Reapply after cleaning if surfaces become visibly soiled Quat-sensitive facilities: - Use as direct replacement for quat-based sanitizers at all touchpoints - Maintain spray bottles at each workstation for continuous access - Document usage for audit trail compliance Storage: - Store at room temperature - Keep away from open flame (alcohol-based)

Direct spray application

Surface sanitation: • Spray surface until thoroughly wet • Allow to air dry (no wiping or rinsing needed) • Reapply after cleaning if surfaces become visibly soiled Poultry house biosecurity: • Spray equipment, door handles, and control panels between house entries • Use at entry points as part of your biosecurity protocol • Pair with boot dips for comprehensive pathogen control Storage: • Store at room temperature • Keep away from open flame (alcohol-based)

Pre-moistened wipe

Surface sanitation: • Pull one wipe from canister • Wipe surface thoroughly until visibly wet • Allow to air dry — no rinsing needed • Discard used wipe (do not flush) High-traffic touchpoints: • Wipe door handles, light switches, and control panels at shift changes • Stage canisters at house entry points, truck cabs, and break rooms • Replace canister lid tightly between uses to prevent dry-out Storage: • Store at room temperature • Keep canister sealed when not in use • Keep away from open flame (alcohol-based)

Dilution and spray, foam, or CIP application

General surface sanitation (food-contact): - Dilute per facility SOP and EPA label directions - Apply to pre-cleaned surfaces - Allow required contact time per label - No potable water rinse required at approved concentrations CIP (Clean-in-Place) systems: - Add to final sanitizing rinse per system specifications - Monitor concentration with PAA test strips - Effective in both ambient and cold-water CIP cycles Storage and handling: - Store in original container at room temperature - Avoid contact with metals (corrosive at concentrate strength) - Use appropriate PPE when handling concentrate

Water line

Water-line delivery: • General use: 1 oz per gallon of drinking water • Stress events (weaning, transport): 2 oz per gallon for 3-5 days • Maintenance: 1 oz per gallon, 3-4 days per week Tips: • Start with lower concentration and increase gradually • Best results when introduced before stress events • Compatible with most water-line systems • Shake well before use — settling is normal for raw ACV

Spray bottle (RTU) or pump sprayer

Indoor use: • Spray directly on insects or along baseboards, window frames, and entry points • Reapply as needed — safe for frequent use in living spaces Outdoor / garden use: • Spray foliage, garden beds, and perimeter areas • Apply in early morning or evening for best results • Reapply after heavy rain Farm / barn use: • Spray animal housing, fence lines, and high-traffic pest areas • Safe around livestock and poultry

Pour into the vault after pumping

Standard maintenance (per 1,000-gallon vault): • Add 2.5 gallons of PORT with 15 gallons of water • Apply when the vault has been freshly pumped • Let the vault fill to capacity — no additional product needed between pumps High-traffic periods: • Add 1 gallon of PORT between cleanouts, or as needed • Add extra water if solids begin to exceed the water line Tip: • Best results come from dosing a freshly pumped vault so the biology establishes before heavy use

Pour into toilet and flush

Monthly maintenance: • Pour one bottle into any toilet • Flush twice • Best applied before bed (gives bacteria 8+ hours of low-flow time to colonize) New system or heavy use: • Use 2 bottles the first month to establish bacterial population • Return to 1 bottle per month after Tips: • Avoid pouring bleach or antibacterial cleaners down drains for 24 hours after treatment • Consistent monthly use produces the best long-term results • Works in systems of all sizes — one bottle treats up to 1,500 gallons

Pour into drain, soak overnight, rinse — no foaming applicator needed for household drains

Active drain fly infestation: • 1 oz per affected drain • Pour in at night and let it sit undisturbed 8+ hours • Rinse with hot water in the morning • Repeat weekly for the first month Why four weeks matters: • Eggs are already in the pipe when you start • The drain fly life cycle runs 8–24 days • A single treatment kills the current generation and misses the next • Weekly for a month outlasts the full cycle Commercial floor drains and grease traps: • Increase to 2–4 oz per drain, weekly • Bulk packaging available for multi-site facility programs Maintenance: • 1 oz per drain, monthly, once the infestation is cleared

Dosatron / 1:128 medicator (water lines), pump sprayer, power washer, or foam applicator

Continuous water-line sanitation (birds present): • Inject through a 1:128 medicator (e.g., Dosatron) using ZeroPoint concentrate as-is • Target 2–4 ppm free chlorine (HOCl) at the medicator outlet • Verify with ORP at the far-end nipple — target ≥650 mV (700+ mV ideal) • Maintain dosed-water pH between 5.0–6.5 for HOCl-dominant chemistry Between-flock water-line cleaning (empty house): • Drain lines completely • Fill with ZeroPoint at 100–200 ppm • 30-minute minimum contact time — do not flush early • Drain, fresh-water flush, and resume normal injection • Verify with full 3-point ORP check within 24 hours Poultry house cleanout (surfaces): • Remove litter and loose organic material • Mix ZeroPoint per label dilution rate • Apply to all surfaces: floors, walls, ceiling, equipment • Allow contact time per EPA label instructions • Ventilate before placing new bedding Livestock facilities: • Remove visible organic material • Apply diluted solution to all surfaces • Allow full contact time before rinsing if required Food processing / equipment: • Follow EPA label for food-contact and non-food-contact surface rates • Rinse food-contact surfaces after disinfection Important: • Always follow EPA label directions for dilution rates and contact times • Wear appropriate PPE during application • Verify efficacy with ORP/pH at the point of consumption — ppm at the medicator does not equal ppm at the bird